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lab n a plvx tetone puro gfp addgene plasmid  (Addgene inc)


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    Structured Review

    Addgene inc lab n a plvx tetone puro gfp addgene plasmid
    Lab N A Plvx Tetone Puro Gfp Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plvx+tetone+puro+gfp/lentiGuide-Puro+(Plasmid+%2352963)/pm41575852-715-21-24
    Average 96 stars, based on 1527 article reviews
    lab n a plvx tetone puro gfp addgene plasmid - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Proteomic analysis links truncated tau to lysosome motility, autophagy, and endo‐lysosomal dysfunction
    Article Snippet: .. At this point, the cells were transfected with the plasmids of interest (pLVX‐TetOne‐Puro—[Tau35‐HA, Tau35‐mKO 2 , mKO 2 ‐Tau35, Avi‐FL tau, FL tau‐eGFP, and eGFP‐FL tau]) generated by GenScript or pLVX‐TetOne‐Puro‐GFP (Addgene plasmid no. 171123; Addgene_171123) using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Catalog No.: L3000015) according to manufacturer's instructions. .. Approximately 48 h after transfection, puromycin dihydrochloride (2 μg/mL, Thermo Fisher Scientific, Catalog No.: A1113803) was added to the culture medium to initiate selection.

    Article Title: Proteomic Analysis Links Truncated Tau to Lysosome Motility, Autophagy and Endo-lysosomal Dysfunction
    Article Snippet: .. At this point, the cells were transfected with the plasmids of interest [pLVX-TetOne-Puro - (Tau35-HA, Tau35-mKO 2 , mKO 2 -Tau35, Avi-FL tau, FL tau-eGFP and eGFP-FL tau) generated by GenScript or pLVX-TetOne-Puro-GFP (Addgene plasmid # 171123; http://n2t.net/addgene:171123;RRID:Addgene_171123 ) ] using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Cat# L3000015) according to manufacturer’s instructions. .. Approximately 48 hours post-transfection, puromycin dihydrochloride (2 μg/ml, Thermo Fisher Scientific, Cat# A1113803) was added to the culture medium to initiate selection.

    Generated:

    Article Title: Proteomic analysis links truncated tau to lysosome motility, autophagy, and endo‐lysosomal dysfunction
    Article Snippet: .. At this point, the cells were transfected with the plasmids of interest (pLVX‐TetOne‐Puro—[Tau35‐HA, Tau35‐mKO 2 , mKO 2 ‐Tau35, Avi‐FL tau, FL tau‐eGFP, and eGFP‐FL tau]) generated by GenScript or pLVX‐TetOne‐Puro‐GFP (Addgene plasmid no. 171123; Addgene_171123) using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Catalog No.: L3000015) according to manufacturer's instructions. .. Approximately 48 h after transfection, puromycin dihydrochloride (2 μg/mL, Thermo Fisher Scientific, Catalog No.: A1113803) was added to the culture medium to initiate selection.

    Article Title: Suppression of stress granule formation is a vulnerability imposed by mutant p53
    Article Snippet: .. Hep3B cells expressing an inducible wtp53 (iwtp53) was generated by infecting a doxycycline-inducible wtp53 vector, in which the pLVX-tetOne-puro-GFP (171123, Addgene) vector was used to replace GFP with human p53 cDNA, followed by puromycin selection. ..

    Article Title: Proteomic Analysis Links Truncated Tau to Lysosome Motility, Autophagy and Endo-lysosomal Dysfunction
    Article Snippet: .. At this point, the cells were transfected with the plasmids of interest [pLVX-TetOne-Puro - (Tau35-HA, Tau35-mKO 2 , mKO 2 -Tau35, Avi-FL tau, FL tau-eGFP and eGFP-FL tau) generated by GenScript or pLVX-TetOne-Puro-GFP (Addgene plasmid # 171123; http://n2t.net/addgene:171123;RRID:Addgene_171123 ) ] using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Cat# L3000015) according to manufacturer’s instructions. .. Approximately 48 hours post-transfection, puromycin dihydrochloride (2 μg/ml, Thermo Fisher Scientific, Cat# A1113803) was added to the culture medium to initiate selection.

    Article Title: Suppression of stress granule formation is a vulnerability imposed by mutant p53.
    Article Snippet: .. Hep3B cells expressing an inducible wtp53 (iwtp53) was generated by infecting a doxycycline-inducible wtp53 vector, in which the pLVX-tetOne-puro-GFP (171123, Addgene) vector was used to replace GFP with human p53 cDNA, followed by puromycin selection. ..

    Plasmid Preparation:

    Article Title: Proteomic analysis links truncated tau to lysosome motility, autophagy, and endo‐lysosomal dysfunction
    Article Snippet: .. At this point, the cells were transfected with the plasmids of interest (pLVX‐TetOne‐Puro—[Tau35‐HA, Tau35‐mKO 2 , mKO 2 ‐Tau35, Avi‐FL tau, FL tau‐eGFP, and eGFP‐FL tau]) generated by GenScript or pLVX‐TetOne‐Puro‐GFP (Addgene plasmid no. 171123; Addgene_171123) using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Catalog No.: L3000015) according to manufacturer's instructions. .. Approximately 48 h after transfection, puromycin dihydrochloride (2 μg/mL, Thermo Fisher Scientific, Catalog No.: A1113803) was added to the culture medium to initiate selection.

    Article Title: Proteomic Analysis Links Truncated Tau to Lysosome Motility, Autophagy and Endo-lysosomal Dysfunction
    Article Snippet: .. At this point, the cells were transfected with the plasmids of interest [pLVX-TetOne-Puro - (Tau35-HA, Tau35-mKO 2 , mKO 2 -Tau35, Avi-FL tau, FL tau-eGFP and eGFP-FL tau) generated by GenScript or pLVX-TetOne-Puro-GFP (Addgene plasmid # 171123; http://n2t.net/addgene:171123;RRID:Addgene_171123 ) ] using Lipofectamine 3000 reagent (Thermo Fisher Scientific, Cat# L3000015) according to manufacturer’s instructions. .. Approximately 48 hours post-transfection, puromycin dihydrochloride (2 μg/ml, Thermo Fisher Scientific, Cat# A1113803) was added to the culture medium to initiate selection.

    Polymerase Chain Reaction:

    Article Title: NEGR1 deficiency disrupts lipid metabolism and steroidogenesis in Leydig cells, linking testosterone to behavior
    Article Snippet: .. The PCR fragment was then assembled with the pLVX-TetOne backbone obtained from pLVX-TetOne-puro-GFP (#171123; Addgene) ( ). ..

    Expressing:

    Article Title: Suppression of stress granule formation is a vulnerability imposed by mutant p53
    Article Snippet: .. Hep3B cells expressing an inducible wtp53 (iwtp53) was generated by infecting a doxycycline-inducible wtp53 vector, in which the pLVX-tetOne-puro-GFP (171123, Addgene) vector was used to replace GFP with human p53 cDNA, followed by puromycin selection. ..

    Article Title: Suppression of stress granule formation is a vulnerability imposed by mutant p53.
    Article Snippet: .. Hep3B cells expressing an inducible wtp53 (iwtp53) was generated by infecting a doxycycline-inducible wtp53 vector, in which the pLVX-tetOne-puro-GFP (171123, Addgene) vector was used to replace GFP with human p53 cDNA, followed by puromycin selection. ..

    Selection:

    Article Title: Suppression of stress granule formation is a vulnerability imposed by mutant p53
    Article Snippet: .. Hep3B cells expressing an inducible wtp53 (iwtp53) was generated by infecting a doxycycline-inducible wtp53 vector, in which the pLVX-tetOne-puro-GFP (171123, Addgene) vector was used to replace GFP with human p53 cDNA, followed by puromycin selection. ..

    Article Title: Suppression of stress granule formation is a vulnerability imposed by mutant p53.
    Article Snippet: .. Hep3B cells expressing an inducible wtp53 (iwtp53) was generated by infecting a doxycycline-inducible wtp53 vector, in which the pLVX-tetOne-puro-GFP (171123, Addgene) vector was used to replace GFP with human p53 cDNA, followed by puromycin selection. ..

    other:

    Article Title: Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies
    Article Snippet: pLVX-TetOne-Puro-GFP , Addgene , Cat. No. 171123; RRID:Addgene_171123.



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